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iColor Fluoro-Chromatic Cytometer

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iColor® Fluoro-Chromatic Imaging Cytometer




  • Fluoro-Chromatic Imaging Laser-Scanning Cytometer
  • Includes Three Integrated Lasers for Simultaneous Chromatic and Fluroescent Measurements
  • Single System for Detailed Quantitative and Morphological Data

Features

  • Simultaneously Measure Chromatic and Fluorescent Staining
  • Detailed Morphological and Quantitative Data Output
  • Analyze Chromatically Stained Specimens
  • Quantify Chromatic Dye Absorption
  • Provides Flexibility in Imaging
  • Corrects for Spectral Overlap
  • Autofocus for Automated Analysis
  • Includes iGen Software
Applications
  • Biochemistry-Like Analysis of Heterogeneous Cell Populations via Total Signal Quantification in Cellular and Tissue Specimens
  • Analysis of Chromatically and Fluorescently Stained Samples Simultaneously or Sequentially
  • Visualize Specimens Conventionally with Either Laser- Scanning Imaging Techniques or a Microscope
  • View Individual Events in the Population Data
  • Reanalyze the Same Cells Under Varying Conditions
  • Measure and Localize Cellular Constituents
  • Detect Molecular Constituents in the Surrounding Environment and Correlate their Presence to Cell Processes
  • Simultaneously Study Cultured Cells at the Individual and Colony Level
  • Perform Automated Analysis on Tissue Sections and Microarrays

The iColor® Fluoro-Chromatic Imaging Cytometer allows simultaneous analysis of fluorescent and chromatically stained specimens. iColor combines the advantages of flow cytometry, fluorescence image analysis, and immunohistochemistry into one complete system. It comes packaged with our iGen software for data acquisition and analysis.

The iColor Cytometer employs three lasers (green, blue, and red) to simultaneously measure both fluorescent light emitted by specimens stained with fluorescent dyes and the light absorbance resulting from the application of chromatic stains to the same specimens (see Figure 1). The resulting data and images provide detailed quantitative and morphological information, which previously would have required the use and expense of two or three different systems.

iColor Cytometer

Figure 1: iColor Schematic

The iColor can perform an initial examination of a tissue microarray (TMA) within 10 minutes, with the option for the observer to review the images. This initial scan documents the visual assessment of the overall array and provides a digital TMA map. During the analysis of the TMA core elements, the iColor derives data from laserscan images and automatically scores the TMA elements. The full walk-away analysis (about 1.5 hours) quantifies data and morphology images produced simultaneously and supports traditional immunohistochemistry (IHC) and fluorescently labeled TMAs. When completed, the iColor produces a fully documented analysis from TMA overview to final results.

Since the specimens under study are on slides, they can be measured repeatedly over time, which is ideal for the study of time-resolved processes such as enzyme kinetics. Data analysis and image processing can be performed either in real time as the scan is progressing or at the post-data acquisition stage, allowing the researcher to modify analysis strategies or explore alternative scenarios. Numerical data are displayed as traditional cytometric histograms or bivariate scattergram plots and can be related in a variety of ways to the image data from which they are derived. Additional options such as Live-Cell Chambers or an Enhanced Blue Cube provide additional data collection abilities.

Specifications
Lasers 440, 532, and 633 nm
Detectors 3 Photomultiplier Tube Fluorescence Detectors (Interchangable Filter Blocks)
3 Solid-State Light-Loss/Forward Scatter Detectors
Fluorescence Channels Cyan (455 - 495 nm)
Yellow (550 - 600 nm)
Long Red (650 - 750 nm)
Accessories Robot-Ready: Run up to 180 Slides
Microscope Olympus IX-71 Microscope Base
Full Microscope Option for Ocular and Camera Visualization
Autofocus Option Fast Laser-Based Autofocus
Visualization High-Resolution Laser-Excited Fluorescence and Laser Light-Loss Imaging,
Laser Scatter Brightfield Imaging, CompuColor™ Imaging System
Virtual Channels and Imaging Processing Perform Image Algebra on Multiple Input Channels
Apply Image Processing Tools and Filters
Specimen Carriers Glass or Plastic Microtiter Plates, Microscope Slides, Petri Dishes, Chamber Slides, and Customer-Defined Formats
Computer Core i7 2600k Processor, 8 GB RAM, 1 TB Hard Drive, LCD Monitor, Windows®7 Operating System
Software iColor™ Cytometric Analysis Software
iBrowser® Data Integration Software
iNovator Application Development Toolkit

Environmental Chamber for Live-Cell Analysis

Environmental Chamber for Live-Cell Analysis

Figure 1: Environmental Chamber on iCys Stage

  • Compatible with iCys Laser-Scanning Cytometer
  • Provides Temperature- and Atmosphere-Controlled Environment
  • Supports Multiple Carrier Types:
    • 35 mm Petri Dishes
    • 50/60 mm Petri Dishes
    • Chambered Cover Glass

The Environmental Chamber for Live-Cell Analysis provides a temperature- and CO2 controlled environment that allows scanning of live cells for extended periods of time on our iCys cytometers. This chamber is ideal for certain assays such as live-cell phagocytosis assay or live HeLa cell growth studies (see Figure 1).

The incubation chamber is maintained at a temperature between 36.5 °C and 37 °C by the incubator control unit, which maintains the gas environment within the chamber at a 5% CO2, 95% air mixture. To maintain precise atmospheric control, 100% CO2 input to the Control Unit is required (CO2 gas tank and tank regulator are not included with the kit). Additionally, multiple sample carriers may be run in the environmental chamber: 35 mm petri dishes, 50/60 mm petri dishes, and chambered cover glass.

Enhanced Blue Cube

Environmental Chamber for Live-Cell Analysis

Figure 2: The Blue Cube within a Cytometer. The Insert Depicts the Light Path

  • Slightly Reduced Bandwidth (430 – 470 nm) Eliminates 488 nm Laser Line Interference
  • Enables 405 and 488 nm Lasers to be Run Simultaneously
  • Compatible with Dual-Channel Absorption/Scatter Detector

The Enhanced Blue Cube, available for the iCys, allows scans using the 405 nm and 488 nm diode lasers to be conducted in a single pass, cutting in half the time required for scanning assays using these lasers individually. The enhanced blue cube is ideal for cell cycle assays where DNA content and chromatin condensation are used to determine the cell cycle phase, and other biomarker expression levels are also assessed.

Combining the Dual-Channel Absorption/Scatter Detector (see Figure 2) with the Enhanced Blue Cube allows the acquisition of 488 shaded relief during a two-laser scan by filtering out the 405 nm component. Either 488 nm or 633 nm light-loss may be acquired along with the 488 shaded relief.

Dual-Channel Laser Light Scatter and Laser Light-Loss Detector

Figure 3: Dual-Channel Detector Diagram. Sensor #1 is Set for Measuring Scatter, While Sensor #2 is Set for Light-Loss.

  • Quantify Chromatic Dye Light-Loss with Two Chromatic Stains in Half the Time
  • Obtain Richer Data in a Single Pass
  • Corrects of Spectral Overlap
  • Combines Fluorescence and Chromatic Analysis

The Dual-Channel Assembly, compatible with the iCys Cytometers, allows for the simultaneous capture by two channels of scatter and/or light-loss data (see Figure 3). The result is decreased throughput time by more than 50% for certain assays and enhanced data sets. When the two channels are used for light-loss, measurements, it is possible to quantify chromatic dye absorption for two dyes in a single pass. When one channel is set for scatter and the other for lightloss, the chromatic dye levels and morphology information can be quantified.

This dual-channel assembly is also capable of correcting for spectral overlap for a single pass scan since both channels of data can be acquired simultaneously. When appropriate, corrections of the chromatic dye images for autofluorescence is also possible because the absorption measurement uses dedicated detectors that are separate from the fluorescencemeasuring photomultiplier tubes. This allows chromatic and fluorescence measurements to be made simultaneously.

Optional Carriers

Chamber Carriers

Figure 4: Compatible Carriers for iCys Cytometers

We offer several addition carrier types for our iCys LSCs and any stage with a microtiter plate footprint (see Figure 4). These carriers easily install into our iCys systems to further accommodate experimental demands. The Chamber Slide Holder is for scanning specimens in chamber slides. This carrier is suitable for live cell analysis. The Petri Dish Holder can scan up to 3 petri dishes in a single run, making it suitable for both fixed- and live-cell analysis. The 4-Slide Holder accommodates up to 4 standard microscope slides simultaneously. This carrier is ideal for applications such as automated tissue and TMA analysis.

ABOUT US

Our Mission is to accelerate the advancement of optical technology for precision measurements and their applications from the table tops of research laboratories to standard use in communication and high technology industries. Our aim is to serve our customers. Our hope is to create a place for highly skilled people in an open environment

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