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iCys Research Imaging Cytometer

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iCys® Research Imaging Cytometer




  • Research Imaging Laser-Scanning Cytometer
  • Includes up to Four Integrated Lasers
  • Compatible with Microscope Optics of Digital Camera for Visualization of Specimen

Features

  • Simultaneously Acquire Multi-Fluorescence and Brightfield Laser-Scatter Images from up to 4 Lasers and 6 Multiplexed Light Sensors
  • Displays Widefield, Super-Widefield, and Mosaic Images
  • On-the-Fly Galleries for Viewing Specimens
  • Analysis and Visualization of Specimens in a Variety of Formats for Cell Population, Cell Colonies, Tissue, and Tissue Array Analysis
  • Motorized Stage Provides Precise Localization for Cells and Tissue Events
  • Autofocus for Automated Analysis
  • Interfaces Easily with Peripheral Microscopy Devices
  • Includes iGen Software

 

Applications
  • Biochemistry-Like Analysis of Heterogeneous Cell Populations via Total Signal Quantification in Cellular and Tissue Specimens
  • Analysis of Chromatically and Fluorescently Stained Samples Simultaneously or Sequentially
  • Visualize Specimens Conventionally with Either Laser- Scanning Imaging Techniques or a Microscope
  • View Individual Events in the Population Data
  • Reanalyze the Same Cells Under Varying Conditions
  • Measure and Localize Cellular Constituents
  • Detect Molecular Constituents in the Surrounding Environment and Correlate their Presence to Cell Processes
  • Simultaneously Study Cultured Cells at the Individual and Colony Level
  • Perform Automated Analysis on Tissue Sections and Microarrays

The iCys® Research Imaging Cytometer combines the technological advances of Laser Scanning Cytometry (LSC) and iCyte instruments to provide the ability to visualize the specimen through microscope optics or with a digital camera. It houses four lasers that are available in five different wavelengths (see Specs tab for more details) for laser-spot scanning illumination. The detection system, which combines PMTs for fluorescence detection and photodiodes for absorbance and scatter detection, offers excellent quantitative cytometric performance and imaging capabilities. This system provides fully automated operation for analysis of cellular and tissue samples that have been stained with fluorescent or chromatic dyes. Fluorescent excitation, laser light absorbance by chromatic dyes, and scatter signals may all be acquired simultaneously. The iCys comes packaged with our iGen software for data acquisition and analysis.

iCyte Cytometer

Figure 1: iCys Schematic

The combination of analytic software advances with microscope features provides the iCys with the ability to automatically move the microscope stage to another region of interest after scanning and analysis are complete. The event can then be viewed using the microscope optics, while simultaneously viewing the laser scan images. The user may further utilize any number of microscope accessories available, including optomechanical devices for micromanipulation and cell capture. The iCys displays the 3D morphology of the specimen with protocol-driven settings for variable angle laser scatter along with absorbance direction. The variable resolution scanning yields the ability to scan larger areas for higher throughput analysis. This produces the best resolution of the analysis without compromising sensitivity. The iCys also combines scanned imaging with live visualization.

Since the specimens under study are on slides, they can be measured repeatedly over time, which is ideal for the study of time-resolved processes such as enzyme kinetics. Data analysis and image processing can be performed either in real time as the scan is progressing or at the post-data acquisition stage, allowing the researcher to modify analysis strategies or explore alternative scenarios. Numerical data are displayed as traditional cytometric histograms or bivariate scattergram plots and can be related in a variety of ways to the image data from which they are derived. Additional options such as Live-Cell Chambers or an Enhanced Blue Cube provide additional data collection abilities.

 

Specifications
Lasers (Up to 4) Violet (405 nm)
Blue (488 nm)
Green (532 nm)
Yellow (561 nm)
Red (633 nm)
Detectors 4 Photomultiplier Tube Fluorescence Detectors (Interchangable Filter Blocks)
2 Photodiodes for Light-Loss/Scatter Detectors
Emission Detection Options Blue (445 - 485 nm)
Green (515 - 585 nm)
Orange (565 - 585 nm)
Red (600 - 635 nm)
Crimson (650 - 700 nm)
Near-Infrared (750 - 800 nm)
Data Channels 6 Data Channels/Pass Plus Ulimited Programmable Virtual Channels
(Multi-Pass Scans Extend the Number of Acquired Data Channels)
Microscope Olympus IX-51 Series Microscope Base
Autofocus Variable-Resolution Laser Scan Imaging (4, 10, 20, 40, or 60 Times Objective Lens Magnification and User-Selectable Rate of Stage Motion
Visualization High-Resolution Laser Scan Imaging with CompuColor™ and Laser Scatter Brightfield Imaging
Specimen Carriers Glass or Plastic Microtiter Plates, Microscope Slides, Petri Dishes, Camber Slides, and Proprietary Carriers
Computer Core i7 2600k Processor, 8 GB RAM, 1 TB HD, LCD Monitor, Windows® 7 OS
Software iCys™ Cytometric Analysis Software
iBrowser® Data Integration Software
iNovator Application Development Toolkit
 

The iCys Cytometer can support up to four lasers to significantly expand the variety of dyes that are compatible with the systems. The fourth laser option comes with either a 532 nm or 561 nm laser (see configuration tables below).

To accommodate the additional laser, the standard emission filter cubes have been reformulated to allow for efficient detection across the emission bands located between the laser lines. This opens up the option for the researcher to utilize various combinations of laser lines and emission bands in single- or multiple-pass scanning. The filtering of absorption and forward scatter detection channels has also been extended. This yields the ability to detect the effect of chromatic stains and sample morphology on each laser line in both single- or multiple-pass scanning without the need for user intervention.

Environmental Chamber for Live-Cell Analysis

Environmental Chamber for Live-Cell Analysis

Figure 1: Environmental Chamber on iCys Stage

  • Compatible with iCys Laser-Scanning Cytometer
  • Provides Temperature- and Atmosphere-Controlled Environment
  • Supports Multiple Carrier Types:
    • 35 mm Petri Dishes
    • 50/60 mm Petri Dishes
    • Chambered Cover Glass

The Environmental Chamber for Live-Cell Analysis provides a temperature- and CO2 controlled environment that allows scanning of live cells for extended periods of time on our iCys cytometers. This chamber is ideal for certain assays such as live-cell phagocytosis assay or live HeLa cell growth studies (see Figure 1).

The incubation chamber is maintained at a temperature between 36.5 °C and 37 °C by the incubator control unit, which maintains the gas environment within the chamber at a 5% CO2, 95% air mixture. To maintain precise atmospheric control, 100% CO2 input to the Control Unit is required (CO2 gas tank and tank regulator are not included with the kit). Additionally, multiple sample carriers may be run in the environmental chamber: 35 mm petri dishes, 50/60 mm petri dishes, and chambered cover glass.

Enhanced Blue Cube

Environmental Chamber for Live-Cell Analysis

Figure 2: The Blue Cube within a Cytometer. The Insert Depicts the Light Path

  • Slightly Reduced Bandwidth (430 – 470 nm) Eliminates 488 nm Laser Line Interference
  • Enables 405 and 488 nm Lasers to be Run Simultaneously
  • Compatible with Dual-Channel Absorption/Scatter Detector

The Enhanced Blue Cube, available for the iCys, allows scans using the 405 nm and 488 nm diode lasers to be conducted in a single pass, cutting in half the time required for scanning assays using these lasers individually. The enhanced blue cube is ideal for cell cycle assays where DNA content and chromatin condensation are used to determine the cell cycle phase, and other biomarker expression levels are also assessed.

Combining the Dual-Channel Absorption/Scatter Detector (see Figure 2) with the Enhanced Blue Cube allows the acquisition of 488 shaded relief during a two-laser scan by filtering out the 405 nm component. Either 488 nm or 633 nm light-loss may be acquired along with the 488 shaded relief.

Dual-Channel Laser Light Scatter and Laser Light-Loss Detector

Figure 3: Dual-Channel Detector Diagram. Sensor #1 is Set for Measuring Scatter, While Sensor #2 is Set for Light-Loss.

  • Quantify Chromatic Dye Light-Loss with Two Chromatic Stains in Half the Time
  • Obtain Richer Data in a Single Pass
  • Corrects of Spectral Overlap
  • Combines Fluorescence and Chromatic Analysis

The Dual-Channel Assembly, compatible with the iCys Cytometers, allows for the simultaneous capture by two channels of scatter and/or light-loss data (see Figure 3). The result is decreased throughput time by more than 50% for certain assays and enhanced data sets. When the two channels are used for light-loss, measurements, it is possible to quantify chromatic dye absorption for two dyes in a single pass. When one channel is set for scatter and the other for lightloss, the chromatic dye levels and morphology information can be quantified.

This dual-channel assembly is also capable of correcting for spectral overlap for a single pass scan since both channels of data can be acquired simultaneously. When appropriate, corrections of the chromatic dye images for autofluorescence is also possible because the absorption measurement uses dedicated detectors that are separate from the fluorescencemeasuring photomultiplier tubes. This allows chromatic and fluorescence measurements to be made simultaneously.

Optional Carriers

Chamber Carriers

Figure 4: Compatible Carriers for iCys Cytometers

We offer several addition carrier types for our iCys LSCs and any stage with a microtiter plate footprint (see Figure 4). These carriers easily install into our iCys systems to further accommodate experimental demands. The Chamber Slide Holder is for scanning specimens in chamber slides. This carrier is suitable for live cell analysis. The Petri Dish Holder can scan up to 3 petri dishes in a single run, making it suitable for both fixed- and live-cell analysis. The 4-Slide Holder accommodates up to 4 standard microscope slides simultaneously. This carrier is ideal for applications such as automated tissue and TMA analysis.

ABOUT US

Our Mission is to accelerate the advancement of optical technology for precision measurements and their applications from the table tops of research laboratories to standard use in communication and high technology industries. Our aim is to serve our customers. Our hope is to create a place for highly skilled people in an open environment

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Tel               +966 35823441
Fax               +966 35823441
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